Antiosteoporotic effect of sequential extracts and freezedried juice of Cissus quadrangularis L. in ovariectomized mice

Thanika Pathomwichaiwat 1 , Wisuda Suvitayavat 2 , Achariya Sailasuta 3 , Pawinee Piyachaturawat 4 , Noppamas Soonthornchareonnon 5  and Sompop Prathanturarug 1
  • 1 Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
  • 2 Department of Physiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand
  • 3 Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
  • 4 Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
  • 5 Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand

Abstract

Background: Osteoporosis becomes a major health problem in aging populations, and this disease increases the risk of bone fractures, leading to disability and mortality. Cissus quadrangularis L. (CQ) has been reported to have beneficial effects on bone metabolism; however, there has been no investigation to identify the active compounds responsible for this activity.

Objective: Sequential extracts (hexane, dichloromethane, ethanol, and water) and freeze-dried CQ juice were investigated to determine their effects on bone metabolism in an ovariectomized (ovx) mouse model.

Methods: Six-week-old ICR mice were divided into eight groups: sham-operated, ovx-control, estradiol (E2)- treated and five CQ-treated ovx-mouse groups (n = 3). The CQ extracts were orally administered at a dose equivalent to 5g of crude powder/kg/day for 8 weeks. Bone mineral densities (BMD) of the femur and tibia, serum levels of osteocalcin (bone formation marker) and TRAP5b (bone resorption marker), and histomorphological change of lumbar spine were determined at the end of the experiment.

Results: The BMD of the femur and tibia in the hexane-treated group were elevated to the same level as those of sham-operated group. This BMDs correlated with restoration of the trabecular bone of the lumbar spine, which was only observed in the hexane-treated group. These results were also supported by the lowest serum levels of osteocalcin and TRAP5b observed in this group, compared to the ovx-control and E2-treated groups, representing a decrease in the bone turnover rate. Neither signs of abnormality nor pathological changes of internal organs were observed after the experiment.

Conclusion: The hexane extract possessed antiosteoporotic activity in ovariectomized mice without any toxicity throughout the experiment. Therefore, the hexane extract is the most interesting for further bioassay-guided purification of pharmacologically active compounds.

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  • 1. Bliuc D, Nguyen ND, Milch VE, Nguyen TV, Eisman JA, Center JR. Mortality risk associated with lowtrauma osteoporotic fracture and subsequent fracture in men and women. JAMA. 2009; 301:513-21.

  • 2. Raisz LG. Physiology and pathophysiology of bone remodeling. Clin Chem. 1999; 45:1353-8.

  • 3. Gunta KE. Disorders of musculoskeletal function: developmental and metabolic disorders. In: Porth CM, Matfin G, editors. Pathophysiology: concepts of altered health states 8th ed. Philadelphia: Lippincott Williams & Wilkins; 2009. p. 1493-518.

  • 4. Halleen JM, Ylipahkala H, Alatalo SL, Janckila AJ, Heikkinen JE, Suominen H, et al. Serum tartrateresistant acid phosphatase 5b, but not 5a, correlates with other markers of bone turnover and bone mineral density. Calcif Tissue Int. 2002; 71:20-5.

  • 5. Szulc P, Delmas PD. Biochemical markers of bone turnover in osteoporosis. In: Marcus R, Feldman D, Nelson DA, Rosen CJ, editors. Osteoporosis 3rd ed. San Diego: Academic Press; 2008. p. 1519-45.

  • 6. Smitinand T. Thai Plant Names. Revised ed. Bangkok: The Forest Herbarium, Royal Forest Department; 2001.

  • 7. Williamson EM. Major herbs of Ayuraveda. London: Churchill Livingstone; 2002.

  • 8. Chopra SS, Patel MR, Gupta LP, Datta IC. Studies on Cissus quadrangularis in experimental fracture repair: effect on chemical parameters in blood. Indian J Med Res. 1975; 63:824-8.

  • 9. Prasad GC, Udupa KN. Effect of Cissus quadrangularis on the healing of cortisone treated fractures. Indian J Med Res. 1963; 51:667-76.

  • 10. Singh LM, Udupa KN. Studies on Cissus quadrangularis in fracture by using phosphorus 32-part III. Indian J Med Sci. 1962; 16:926-31.

  • 11. Udupa KN, Arnikar HJ, Singh LM. Experimental studies of the use of Cissus quadrangularis in healing of fractures (part II). Indian J Med Sci. 1961; 15:551-7.

  • 12. Udupa KN, Prasad GC. Biomechanical and calcium-45 studies on the effect of Cissus quadrangularis in fracture repair. Indian J Med Res. 1964; 52:480-7.

  • 13. Udupa KN, Prasad GC. Further studies on the effect of Cissus quadrangularis in accelerating fracture healing. Indian J Med Res. 1964; 52:26-35.

  • 14. Shirwaikar A, Khan S, Malini S. Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat. J Ethnopharmacol. 2003; 89:245-50.

    • Crossref
    • Export Citation
  • 15. Potu BK, Rao MS, Nampurath GK, Chamallamudi MR, Prasad K, Nayak SR, et al. Evidence-based assessment of antiosteoporotic activity of petroleum-ether extract of Cissus quadrangularis Linn. on ovariectomyinduced osteoporosis. Ups J Med Sci. 2009; 114: 140-8.

  • 16. Potu BK, Rao MS, Nampurath GK, Chamallamudi MR, Nayak SR, Thomas H. Anti-osteoporotic activity of the petroleum ether extract of Cissus quadrangularis Linn. in ovariectomized Wistar rats. Chang Gung Med J. 2010; 33:252-7.

  • 17. Potu BK, Rao MS, Kutty NG, Bhat KM, Chamallamudi MR, Nayak SR. Petroleum ether extract of Cissus quadrangularis (Linn) stimulates the growth of fetal bone during intra uterine developmental period: a morphometric analysis. Clinics (Sao Paulo). 2008; 63: 815-20.

    • Crossref
    • Export Citation
  • 18. Udupa KN, Prasad GC. Cissus quadrangularis in healing of fractures. A clinical study. J indian Med Assoc. 1962; 38:590-3.

  • 19. Parisuthiman D, Singhatanadgit W, Dechatiwongse T, Koontongkaew S. Cissus quadrangularis extract enhances biomineralization through up-regulation of MAPK-dependent alkaline phosphatase activity in osteoblasts. In Vitro Cell Dev Biol Anim. 2009; 45: 194-200.

    • Crossref
    • Export Citation
  • 20. Potu BK, Bhat KM, Rao MS, Nampurath GK, Chamallamudi MR, Nayak SR, et al. Petroleum ether extract of Cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis. Clinics (Sao Paulo). 2009; 64:993-8.

    • Crossref
    • Export Citation
  • 21. Jainu M, Devi CSS. Gastroprotective action of Cissus quadrangularis extract against NSAID induced gastric ulcer: role of proinflammatory cytokines and oxidative damage. Chem-Biol Interact. 2006; 161:262-70.

  • 22. Jainu M, Mohan KV, Devi CSS. Protective effect of Cissus quadrangularis on neutrophil mediated tissue injury induced by aspirin in rats. J Ethnopharmacol. 2006; 104:302-5.

    • Crossref
    • Export Citation
  • 23. Jainu M, Mohan KV, Devi CSS. Gastroprotective effect of Cissus quadrangularis extract in rats with experimentally induced ulcer. Indian J Med Res. 2006; 123:799-806.

  • 24. Attawish A, Chavalittumrong P, Chivapat S, Chuthaputti A, Rattanajarasroj S, Punyamong S. Subchronic toxicity of Cissus quadrangularis Linn. Songklanakarin J Sci Technol. 2002; 24:39-51.

  • 25. Viswanatha Swamy AHM, Thippeswamy AHM, Manjula DV, Mahendra Kumar CB. Some neuropharmacological effects of the methanolic root extract of Cissus quadrangularis in mice. Afr J Biomed Res. 2006; 9:69-75.

  • 26. Federer WT. Experimental design: theory and application. Calcutta: Oxford & IBH; 1955.

  • 27. Ke H. In vivo characterization of skeletal phenotype of genetically modified mice. J Bone Miner Metab. 2005; 23:84-9.

    • Crossref
    • Export Citation
  • 28. Pogoda P, Priemel M, Schilling AF, Gebauer M, Catala-Lehnenf P, Barvencik F, et al. Mouse models in skeletal physiology and osteoporosis: experiences and data on 14839 cases from the Hamburg Mouse Archives. J Bone Miner Metab. 2005; 23:97-102.

    • Crossref
    • Export Citation
  • 29. Mödder UI, Riggs BL, Spelsberg TC, Fraser DG, Atkinson EJ, Arnold R, et al. Dose-response of estrogen on bone versus the uterus in ovariectomized mice. Eur J Endocrinol. 2004; 151:503-10.

  • MAPK-dependent alkaline phosphatase activity in osteoblasts. In Vitro Cell Dev Biol Anim. 2009; 45: 194-200.

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