An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Open access


An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L-1 BA and 0.05 mg L-1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L-1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species

BACH A, and PAWŁOWSKA B. 2003. Somatic embryogenesis in Gentiana pneumonanathe L. Acta BiologicaCracoviensia Series Botanica 45(2): 79-86.

BANERJEE S, HAIDER F, BAGCHI GD, and SAMAD A. 2010. Regeneration of phytoplasma-free Artemisia roxburghiana Besser var. purpurascens (Jacq.) Hook. plants using apical meristem culture. Plant Cell Tissue andOrgan Culture 103:189-196.

BASKARAN P, and JAYABALAN N. 2005. An efficient micropropagation system for Eclipa alba - a valuable medicinal herb. In Vitro Cellular and Developmental Biology -Plant 41: 532-539.

BASKARAN P, RAJA RAJEWARI B, and JAYABALAN N. 2006. Development of an in vitro regeneration system in sorghum (Sorghum bicolor (L.) Moench) using root transverse thin cell layers (tTCLs). Turkish Journal ofBotany 30: 1-9.

BHATTACHARYA P, DEY S, DAS N, and BHATTACHARYA BC. 1990. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant CellReports 9: 439-442.

BIRMETA G, and WELANDER M. 2004. Efficient micropropagation of Ensete ventricosum applying meristem wounding: a three-step protocol. Plant Cell Reports 23: 277-283.

CHENG ZJ, ZHU SS, GAO XQ, and ZHANG XS. 2010. Cytokinin and auxin regulates WUS induction and inflorescence regeneration in vitro in Arabidopsis. Plant Cell Reports 29: 927-933.

ĆURKOVIĆ PM. 2003. In vitro propagation of Centaurearupestris L. Acta Biologica Cracoviensia SeriesBotanica 45 (2): 127-130.

DEBERGH PC, and MAENE LJ, 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Horticulturae 14: 335-345.

GASPAR T, KEVERS C, FAIVRE-RAMPANT O, CREVECOEUR M, PENEL C, GREPPIN H, and DOMMES J. 2003. Changing concepts in plant hormone action. In Vitro Cellular andDevelopmental Biology - Plant 39: 85-106.

GORDON SP, CHICKARMANE VS, OHNO C, and MEYEROWITZ EM. 2009. Multiple feedback loops through cytokinin signaling control stem cell number within the Arabidopsis shoot meristem. Proceedings of the National Academyof Sciences USA 106: 16529-16534.

HAZARIKA BN. 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae 108: 105-120.

JOSHI M, and DHAR U. 2003. In vitro propagation of Saussurea obvallata (DC.) Edgew. - an endangered ethnoreligious medicinal herb of Himalaya. Plant CellReports 21: 933-939.

KANWAR JK, and KUMAR S. 2008. In vitro propagation of Gerbera. Horticultural Science 35: 35-44.

KAWIAK A, and ŁOJKOWSKA E. 2004. Application of RAPT in the determination of genetic fidelity in micropropagated Drosera plantlets. In Vitro Cellular and DevelopmentalBiology - Plant 40: 592-595.

KAWIAK A, KRÓLICA A, and ŁOJKOWSKA E. 2003. Direct regeneration of Drosera from leaf explants and shoot tips. PlantCell Tissue and Organ Culture 75: 175-178.

KORACH A, JULIANI HR, KAPTEYN J, and SIMON JE. 2002. In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell, Tissue and Organ Culture 69: 79-83.

KOTOV AA, and KOTOVA LM. 2000. The contents of auxins and cytokinins in pea internodes as related to the growth of lateral buds. Journal of Plant Physiology 156: 438-448.

KROMER K, NOWAK T, WOJTUŃ B, and POTURAŁA D. 2000. In vitro propagation of the populations of Drosera intermedia from Izerskie Mountains. Biuletyn OgrodówBotanicznych, Muzeów i Zbiorów 9: 147-152.

MAŁECKA J. 1962. Studies in the mode of reproduction of the diploid endemic species Taraxacum pieninicum Pawł. Acta Biologica Cracoviensia Series Botanica 4(1): 25-42.

MONDAL TK, BHATTACHARYA A, SOOD A, and AHUJA PS. 1998. Micropropagation of tea (Camellia sinensis (L.) O. Kuntze) using Thidiazuron. Plant Growth Regulation 26: 57-61.

MURANYI R, and WRÓBEL I. 2006. Rola narodowego banku nasion w ochronie zagrożonych gatunków roślin Pienińskiego Parku Narodowego. Pieniny - Przyroda iCzłowiek 9: 79-85.

MURASHIGE T, and SKOOG F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 437-497.

PAUDYAL KP, and HAQ N. 2000. In vitro propagation of Pummelo (Citrus grandis L. OSBECK). In Vitro Cellularand Developmental Biology - Plant 36: 511-516.

RYBCZYŃSKI JJ, and MIKUŁA A. 2006. Engagement of biotechnology in the protection of threatened plant species in Poland. Biodiversity Research and Conservation 3-4: 361-368.

SADIK K, ARINAITWE G, SSEBULIBA JM, GIBSON P, LUGOLOBI C, and MUKASA SB. 2012. Proliferation and shoot recovery among the East African highland banana. African CropScience Journal 20: 67-76.

SHANI E, YANAI O, and ORI N. 2006. The role of hormones in shoot apical meristem function. Current Opinion inPlant Biology 9: 484-489.

SKOOG F, and MILLER CO. 1957. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposia of the Society for Experimental Biology 54: 118-130.

SUGIYAMA M. 1999. Organogenesis in vitro. Current Opinion inPlant Biology 2: 61-64.

SURMACZ-MAGDZIAK A, and SUGIER D. 2012. In vitro propagation of Arnica montana L.: an endangered herbal species of great importance to medicine. Acta ScientiarumPolonorum Seria Hortorum Cultus 11(2): 127-140.

TREJGELL A, and TRETYN A. 2011. Shoot multiplication and in vitro rooting of Carlina onopordifolia Basser. ActaBiologica Cracoviensia Series Botanica 53(2): 68-72.

TREJGELL A, BEDNAREK M, and TRETYN A. 2009. Micropropagation of Carlina acaulis L. Acta BiologicaCracoviensia Series Botanica 51(1): 97-103.

TREJGELL A, DĄBROWSKA A, and TRETYN A. 2012. Micropropagation and influence of in vitro culture on development of Cirsium pannonicum (L. f.) Link regenerants. Acta Scientiarum Polonorum Seria HortorumCultus 11(5): 81-90.

TREJGELL A, DOMżALSKA L, SZCZEPANEK D, and TRETYN A. 2010a. In vitro propagation of Leontopodium alpinum L. from different seedling explants. Propagation ofOrnamental Plant 10: 81-87.

TREJGELL A, MICHALSKA M, and TRETYN A. 2010b. Micropropagation of Senecio macrophyllus M. Bieb. Acta Biologica Cracoviensia Series Botanica 52(2): 67-72.

TYBURSKI J, and TRETYN A. 2004. The role of light and polar auxin transport in root regeneration from hypocotyls of tomato seedling cuttings. Plant Growth Regulation 42: 39-48.

WRÓBEL I. 2004. Mniszek pieniński Taraxacum pieninicum Pawł. - gatunek specjalnej troski w Pienińskim Parku Narodowym. Chrońmy Przyrodę Ojczystą 60(2): 11-16.

WRÓBEL I, and ZARZYCKI K. 2008. Taraxacum pieninicum Pawł. Mniszek pieniński. In: Mirek Z, Piękoś-Mirkowa H [eds.], Czerwona Księga Karpat Polskich, RoślinyNaczyniowe, 406-408. Instytut Botaniki im. W. Szafera PAN, Instytut Ochrony Przyrody PAN, Kraków.

ZARZYCKI K. 1976. Małe populacje pienińskich roślin reliktowych i endemicznych, ich zagrożenie i problemy ochrony. Ochrona Przyrody 41: 7-75.

ZARZYCKI K, and SZELĄG Z. 2006. Red list of the vascular plants in Poland. In: Mirek Z, Zarzycki K, Wojewoda W, Szeląg Z [eds.], Red List of Plants and Fungi in Poland, 9-20. W. Szafer Institute of Botany, Polish Academy of Sciences, Cracow.

ZARZYCKI K, WRÓBEL I, and WRÓBEL S. 2001. Taraxacumpieninicum Pawł. Mniszek pieniński. In: Kaźmierczakowa R, Zarzycki K. [eds.], Polska Czerwona KsięgaRoślin, Paprotniki i Rośliny Kwiatowe, 392-393. Instytut Botaniki im. W. Szafera i Instytut Ochrony Przyrody PAN, Kraków.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2016: 0.491
5-year IMPACT FACTOR: 0.787

CiteScore 2016: 0.51

SCImago Journal Rank (SJR) 2016: 0.242
Source Normalized Impact per Paper (SNIP) 2016: 0.264

Ministry of Science and Higher Education: 20 points

Cited By


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 169 169 12
PDF Downloads 53 53 4