Ultrastructural Changes in Maize Leaf Cells Infected with Maize Dwarf Mosaic Virus and Sugarcane Mosaic Virus

Open access

Plant viruses create many changes in the morphology of the plant cell once the infection process has begun. This paper describes and compares the ultrastructural changes induced in maize cells by two isolates of Maize dwarfmosaic virus (MDMV), Spanish (MDMV-Sp) and Polish (MDMV-P), and one isolate of Sugarcane mosaic virus (SCMV) at 10 and 42 days post-inoculation: the concentration and arrangement of virus particles, inclusion bodies associated with infection, and other cytological alterations. The most important difference between maize cells infected with MDMV isolates and with SCMV-P1 was in the form of cytoplasmic cylindrical inclusions. In cells infected with MDMV only typical inclusions such as pinwheels and scrolls were observed, but laminar aggregates were also present in SCMV-infected cells. No virus particles were found in plant cell organelles. Specific virion arrangements occurred in cells infected with MDMV-Sp and SCMV. The most interesting new finding was of specific amorphous inclusions in the cytoplasm of MDMV-Sp-infected cells, which clearly differentiated the two MDMV isolates studied.

ACHON MA, PINNER M, MEDINA V, and LOMONOSSOFF GP. 1996. Biological characteristics of maize dwarf mosaic potyvirus from Spain. European Journal of Plant Pathology 102: 697-705.

CALDER VL, and INGERFELD M. 1990. The roles of the cylindrical inclusion protein of a potyvirus in the induction of vesicles and in cell-to-cell spread. Journal of Structural Biology 105: 62-66.

DE MEJIA MVG, HIEBERT E, and PURCIFULL DE. 1985a. Isolation and partial characterization of the amorphous cytoplasmic inclusions associated with infections caused by two potyviruses. Virology 142: 24-33.

DE MEJIA MVG, HIEBERT E, PURCIFULL DE, THORNBURY DV, and PIRONE TP. 1985b. Identification of potyviral amorphous inclusion protein as a nonstructural, virus-specific protein related to helper component. Virology 142: 34-43.

EDWARDSON JR. 1974. Some properties of the potato virus Y group. Florida Agricultural Experiment Station Monograph Series No.4, University of Florida, Gainesville.

EDWARDSON JR, CHRISTIE RG, and KO NJ. 1984. Potyvirus cylindrical inclusions - subdivision IV. Phytopathology 74 (9): 1111-1114.

FRANCKI RIB, MILNE RG, and HATTA I. 1985. Atlas of Plant Viruses, vol. II. CRC Press, Boca Raton.

FUCHS E. 2004. Sugarcane mosaic. In: Lapierre H and Signoret PA [eds.], Viruses and Virus Diseases of Poaceae (Gramineae), 690-692. INRA, Paris.

GARBACZEWSKA G, CHRZANOWSKA M, and KERLAN C. 1996. Ultrastructural studies in Nicotiana clevelandii and potato plants infected with PVYNTN. Annals of Warsaw Agricultural University-SGGW. Agriculture 31: 15-22.

GILL CC, and CHONG J. 1979. Cytological alterations in cells infected with corn leaf aphid-specific isolates of barley yellow dwarf virus. Phytopathology 69: 363-368.

GORDON DT. 2004. Maize dwarf mosaic. In: Lapierre H and Signoret PA [eds.], Viruses and Virus Diseases of Poaceae (Gramineae) 644-649. INRA, Paris.

KRASS CJ, and FORD RE. 1969. Ultrastructure of corn systemically infected with Maize dwarf mosaic virus. Phytopathology 59: 431-439.

LAWSON RH, HEARON SS, and SMITH FF. 1971. Development of pinwheel inclusions associated with sweet potato russet crack virus. Virology 46: 453-463.

LESEMANN DE. 1988. Cytopathology. In: Milne RG [ed.], The Plant Viruses, vol. 4. The Filamentous Viruses, 179-235. Plenum Press, New York and London.

LESEMANN DE. 1995. Intracellular symptoms. In: Loebenstein G, Lawson RH, and Brunt AA. [eds.], Virus and Virus-like Diseases of Bulb and Flower Crops, 99-116. J. Wiley & Sons, Chichester.

LESEMANN DE. 1999. Virus specific cytological effects in infected plant cells. Phyton 39 (3): 41-45.

LESEMANN DE, KOENIG R, and HEIN A. 1979. Statice virus Y - a virus related to bean yellow mosaic and clover yellow vein viruses. Phytopathologische Zeitschrift 95: 128-139.

LESEMANN DE, SHUKLA DD, TOSIC M, and HUTH W. 1992. Differentiation of the four viruses of the sugarcane mosaic virus subgroup based on cytopathology. Archives of Virology (Suppl. 5): 353-361.

MARTELLI GP, and RUSSO M. 1976. Unusual cytoplasmic inclusions induced by watermelon mosaic virus. Virology 72: 352-362.

MARTELLI GP, and RUSSO M. 1977. Plant virus inclusion bodies. Advances in Virus Research 21: 175-266.

MURANT AF, and ROBERTS IM. 1971. Cylindrical inclusions in coriander leaf cells infected with parsnip mosaic virus. Journal of General Virology 10: 65-70.

PURCIFULL DE, and EDWARDSON JR. 1967. Watermelon mosaic virus: tubular inclusions in pumpkin leaves and aggregates in leaf extracts. Virology 32: 393-401.

ROBERTS IM, WANG D, FINDLAY K, and MAULE AJ. 1998. Ultrastructural and temporal observations of the potyvirus cylindrical inclusions (CIs) show that the CI protein acts transiently in aiding virus movement. Virology 245: 173-181.

RUSSO M, and MARTELLI GP. 1969. Cytology of Gomphrena globosa L. plants infected by Beet mosaic virus (BMV). Phytopathologia Mediterranea 8(2): 65-82.

SHUKLA DD, TOSIC M, JILKA J, FORD RE, TOLER RW, and LANGHAM MAC. 1989. Taxonomy of potyviruses infecting maize, sorghum and sugarcane in Australia and in the United States as determined by reactivities of polyclonal antibodies directed towards virus-specific N-termini of coat proteins. Phytopathology 79: 223-229.

TRZMIEL K. 2009. First report of Sugarcane mosaic virus infecting maize in Poland. Plant Disease 93(10): 1078.

TRZMIEL K, and JEżEWSKA M. 2008. Identification of Maize dwarf mosaic virus in maize in Poland. Plant Disease 92(6): 981.

ZECHMANN B, MULLER M, and ZELLNIG G. 2003. Cytological modifications in zucchini yellow mosaic virus (ZYMV)-infected Styrian pumpkin plants. Archives of Virology 148: 1119-1133.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

Journal Information


IMPACT FACTOR 2016: 0.491
5-year IMPACT FACTOR: 0.787

CiteScore 2016: 0.51

SCImago Journal Rank (SJR) 2016: 0.242
Source Normalized Impact per Paper (SNIP) 2016: 0.264

Ministry of Science and Higher Education: 20 points

Cited By

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 140 140 15
PDF Downloads 57 57 10