Do Arbuscular Mycorrhizal Fungi Affect Metallothionein Mt2 Expression In Brassica Napus L. Roots?

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Arbuscular mycorrhizal fungi are the most widespread root fungal symbionts, forming associations with the vast majority of plant species. Ectomycorrhizal development alters gene expression in plant symbionts. In this work we examined the effect of arbuscular mycorrhizal fungi spores on the growth and development of Brassica and on the expression of BnMT2 in winter rape. In a pot experiment, rape seedlings growing on different types of sterile and nonsterile soils were inoculated simultaneously with mycorrhizal fungi spores of Acaulospora longula, Glomus geosporum, Glomus mosseae and Scutellospora calospora. As compared with control plants growing in the absence of spores, ten-week-old seedlings of Brassica napus L. in sterile soil inoculated with arbuscular spores had longer shoots and higher fresh biomass of above-ground plant parts. In other types of substrates enriched with mycorrhizal fungi spores, the plants were smaller than non-inoculated plants. The presence of AMF spores stimulated the elongation growth of hypocotyls in both analyzed substrates. BnMT2 expression was highest in plants growing on the sterile substrate. Generally, the presence of mycorrhizal fungi spores appeared to have an adverse effect on the growth of rape plants.

BłASZKOWSKI J, KOWALCZYK S, and CZERNIAWSKA B. 2006. Acaulospora rehmii and Gigaspora margarita, arbuscular mycorrhizal fungi (Glomeromycota) new for Europe and Poland, respectively. Acta Mycologica 41: 41-48.

BORIE F, RUBIO R, and MORALES A. 2008. Arbuscular mycorrhizal fungi and soil aggregation. Journal of Plant Nutrition and Soil Science 8: 9-18.

CHEN H-J, HOU W-C, YANG C-Y, HUANG D-J, LIU J-S, and LIN YH. 2003. Molecular cloning of two metallothionein-like protein genes with differential expression patterns from sweet potato (Ipomoea batatas) leaves. Journal of Plant Physiology 160: 547-555.

CHOI D, KIM HM, YUN HK, PARK J-A, KIM WT, and BOK SH. 1996. Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L. and its induction by wounding and tobacco mosaic virus infection. Plant Physiology 112: 353-359.

CHOMCZYŃSKI P, and SACCHI N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Analytical Biochemistry 162: 156-159.

CICATELLI A, LINGUA G, TODESCHINI V, BIONDI S, TORRIGIANI P, and CASTIGLIONE S. 2010. Arbuscular mycorrhizal fungi restore normal growth in a white poplar clone grown on heavy metal-contaminated soil, and this is associated with upregulation of foliar metallothionein and polyamine biosynthetic gene expression. Annals of Botany 106 (5): 791-802.

COBBETT C, and GOLDSBROUGH P. 2000. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annual Review of Plant Biology 53: 159-182.

DĄBROWSKA G, KATA A, GOC A, SZECHYŃSKA-HEBDA M, and SKRZYPEK E. 2007. Characteristics of the plant ascorbate peroxidase family. Acta Biologica Cracoviensia Series Botanica 49 (1): 7-17.

DUNAEVA M, and ADAMSKA I. 2001. Identification of genes expressed in response to light stress in leaves of Arabidopsis thaliana using RNA differential display. European Journal of Biochemistry 268: 5521-5529.

GARD N, and CHANDEL S. 2010 Arbuscular mycorrhizal networks: process and functions. A review. Agronomy for Sustainable Development 30: 581-599.

HRYNKIEWICZ K, DĄBROWSKA G, BAUM C, NIEDOJADłO K, and LEINWEBER P. 2012. Interactive and single effects of ectomycorrhiza formation and Bacillus cereus on metallothionein MT1 expression and phytoextraction of Cd and Zn by willows. Water, Air, & Soil Pollution DOI: 10.1007/s11270-011-0915-5.

JANSA J, MOZAFAR A, KUHN G, ANKEN T, RUH R, SANDERS IR, and FROSSARD E. 2003. Soil tillage affect the community structure mycorrhizal fungi in maize roots. Ecological Applications 13 (4): 1164-1176.

KABIR Z, O'HALLORAN IP, and HAMEL C. 1996. The proliferation of fungal hyphae in soils supporting mycorrhizal and non-mycorrhizal plants. Mycorrhiza 6: 477-480.

KHAN IA, AHMAD S, and AYUB N. 2003. Yield and nutrients uptake of Avena sativa as influenced by vesicular arbuscular mycorrhizal (VAM). Asian Journal of Plant Sciences 2 (4): 347-376.

KOSZUCKA AM, and DĄBROWSKA G. 2006. Plant metallothioneins. Advances in Cell Biology 33 (2): 285-302. [In Polish].

MA M, LAU P-S, JIA Y-T, TSANG W-K, LAM SKS, TAM NFY, and WONG Y-S. 2003. The isolation and characterization of type 1 metallothionein (MT) cDNA from a heavy-metaltolerant plant, Festuca rubra cv. Merlin. Plant Science 164: 51-60.

MILLER MH. 2000. Arbuscular mycorrhizae and the phosphorus nutrition of maize: a review of Guelph studies. Canadian Journal of Plant Science 80: 47-52.

MIR G, DOMENECH J, HUGUET G, GUO W-J, GOLDSBROUGH P, ATRIAN S, and MOLINAS M. 2004. A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. Journal of Experimental Botany 55: 2483-2493.

OEHL F, SIEVERDING E, INEICHEN K, RISS E-A, BOLLER T, and WIEMKEN A. 2005. Community structure of arbuscular mycorrhizal fungi at different soil depths in extensively and intensively managed agroecosystems. New Phytologist 165: 273-283.

OEHL F, SIEVERDING E, INEICHEN K, MADER P, BOLLER T, and WIEMKEN A. 2003. Impact of land use intensity on the species diversity of arbuscular mycorrhizal fungi in agroecosystems of Central Europe. Applied and Environmental Microbiology 69: 2816-2824.

OLSEN JK, SCHAEFER JT, EDWARDS DG, HUNTER MN, GALEA VJ, and MULLER LM. 1999. Effects of mycorrhizae, established from an existing intact hyphal network, on the growth response of capsicuum (Capsicuum annuum L.) and tomato (Lycopersicon esculentum Mill.) to five rates of applied phosphorus. Australian Journal of Agricultural Research 50: 223-237.

OUZIAD F, HILDEBRANDT U, SCHMELZER E, and BOTHE H. 2005. Differential gene expressions in arbuscular mycorrhizalcolonized tomato grown under heavy metal stress. Journal of Plant Physiology 162: 634-649.

PELLERIN S, MOLLIER A, MOREL C, and PLENCHETTE C. 2007 Effect of incorporation of Brassica napus L. residues in soils on mycorrhizal fungus colonization of roots and phosphorus uptake by maize (Zea mays L.). European Journal of Agronomy 26: 113-120.

READ DJ. 1999. Mycorrhiza - The state of the art. In: Varma A, Hock B [eds.], Mycorrhizae, 3-34. 2nd ed. Springer, Berlin-Heidelberg-New York.

RILLING MC, WRIGHT SF, and EVINER V. 2002. The role of arbuscular mycorrhizal fungi and glomalin in soil aggregation: comparing effects of five plant species. Plant, Soil and Environment 238: 325-333.

RILLING MC, WRIGHT SF, NICHOLS KA, SCHMIDT WF, and TORN MS. 2001. Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils. Plant, Soil and Environment 233: 167-177.

RIVERA-BECERRIL F, VAN TUINEN D, MARTIN-LAURENT F, METWALLY A, DIETZ K-J, GIANINAZZI S, and GIANINAZZI-PEARSON V. 2005. Molecular changes in Pisum sativum L. roots during arbuscular mycorrhiza buffering of cadmium stress. Mycorrhiza 16: 51-60.

ROBINSON NJ, TOMMEY A.M, KUSKE C, and JACKSON JP. 1993. Plant metallothioneins. Biochemical Journal 295: 1-10.

SAMBROOK J, FRITSCH EF, and MANIATIS T. 1989. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

SCANDALIOS JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101: 7-12.

SCHREINER RP, and KOIDE RT. 1992. Antifungal compounds from the roots of mycotrophic and non-mycotrophic plant species. New Phytologist 123: 99-105.

SMITH S, and READ D. 1997. Mycorrhizal Symbiosis. Academic Press, San Diego, California, USA.

SINGH AN, SINGH AR, REXER K-H, KOST G, and VARMA A. 2003. Root endosymbiont: Piriformospora indica - a boon for orchids. Journal of Orchid Society of India 15: 89-102.

TURAN M and ESRINGU A. 2007. Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn. Plant, Soil and Environment 53: 7-15.

VARMA A, VARMA S, SUDHA A, SAHAY NS, and FRANKEN P. 1999. Piriformospora indica, a cultivable plant growth promoting root endophyte with similarities to arbuscular mycorrhizal fungi. Applied and Environmental Microbiology 65: 2741-2744.

VOIBLET C, DUPLESSIS S, ENCELOT N, and MARTIN F. 2001. Identification of symbiosis-regulated genes in Eucalyptus globules, Pisolithus tinctorius ectomycorrhiza by differential hybridization of arrayed cDNAs. Plant Journal 25: 181-191.

WATRUD LS, KING G, LONDO JP, COLASANTIR R, SMITH BM, WASCHMANN RS, and LEE EH. 2011. Changes in constructed Brassica communities treated with glyphosate drift. Ecological Applications 21: 525-538.

WRIGHT SF, FRANKE-SNYDER M, MORTON JB, and UPADHYAYA A. 1996. Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during activa colonization of roots. Plant, Soil and Environment 181: 193-203.

ZHAO S, LIN Q, QI Y, and DUO L. 2010. Responses of root growth and protective enzymes to copper stress in turfgrass. Acta Biologica Cracoviensia Series Botanica 52 (2): 7-11.

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