Comparison of Growth Responses to Auxin 1-Naphthaleneacetic Acid and the Ethylene Precursor 1-Aminocyclopropane-1-Carboxilic Acid in Maize Seedling Root

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Application of 1-naphthaleneacetic acid (NAA) or 1-aminocyclopropane-1-carboxilic acid (ACC) to maize roots growing in hydroponic solution inhibited root elongation, and increased radial growth, but the responses to those treatments differed in degree. Auxin was more effective than ACC as an elongation inhibitor and root swelling promoter. Whereas NAA fully inhibited elongation and maintained swelling over 48 h, ACC inhibited elongation partially (50%) and only promoted swelling for 24 h. It is well-known that auxin, like ACC, promotes ethylene production, but similar levels of ethylene production reached by means of NAA or ACC treatments did not elicit the same response, the response being always stronger to NAA than to ACC. These results suggest that the effect of auxin on root growth is not mediated by ethylene. Elongation and swelling of roots appear to be inversely related: usually a reduction in elongation was accompanied by corresponding swelling. However, these two processes showed different sensitivities to growth regulators. After 24 h treatment with 0.5 μM NAA or 5 μM ACC, root elongation was inhibited by 90% and 53% respectively, but the same treatments promoted swelling by 187% and 140% respectively. Furthermore, 1 μM ACC was shown to promote inhibition of root elongation without affecting swelling. The ethylene antagonist STS (silver thiosulfate) did not affect elongation in control or NAAtreated roots, but increased ethylene production and swelling. These results indicate that longitudinal and radial expansion could be independently controlled.

ABELES FB, MORGAN PW, and SALTVEIT ME. 1992. Ethylene in Plant Biology. Academic Press, New York.

ALARCÓN MV, LLORET PG, IGLESIAS DJ, TALÓN M, and SALGUERO J. 2009a. Response of maize seedling roots to changing ethylene concentrations. Russian Journal of Plant Physiology 56: 488-494.

ALARCÓN MV, LLORET-SALAMANCA A, LLORET PG, IGLESIAS DJ, TALÓN M, and SALGUERO J. 2009b. Effects of antagonists and inhibitors of ethylene biosynthesis on maize root elongation. Plant Signaling and Behavior 4: 1154-1156.

BUER CS, SUKUMAR P, and MUDAY GK. 2006. Ethylene modulates flavonoid accumulation and gravitropic response in roots of Arabidopsis. Plant Physiology 140: 1384-1396.

CHILLEY PM, CASSON SA, TARKOWSKI P, HAWKINS N, WANG KLC, HUSSEY PJ, BEALE M, ECKER JR, SANDBERG GK, and LINDSEY K 2006. The POLARIS peptide of Arabidopsis regulates auxin transport and root growth via effects on ethylene signaling. Plant Cell 18: 3058-3072.

DE CNODDER T, VISSENBERG K, VAN DER STRAETEN D, and VERBELEN JP. 2005. Regulation of cell length in the Arabidopsis thaliana root by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid: a matter of apoplastic reactions. New Phytology 168: 541-550.

DE KLERK GJ, and HANECAKOVA J. 2008. Ethylene and rooting of mung bean cuttings. The role of auxin induced ethylene synthesis and phase-dependent effects. Plant Growth Regulation 56: 203-209.

DOLAN L. 1997. The role of ethylene in the development of plant form. Journal of Experimental Botany 48: 201-210.

DUGARDEYN J, and VAN DER STRAETEN D. 2008. Ethylene: Inhibitor and stimulator of plant growth. In: Bögre L and Beemster G [eds.], Plant Cell Monographs, 199-221. Springer-Verlag, Berlin Heildeberg.

ELIASSON L, BERTELL G, and BOLANDER E. 1989. Inhibitory action of auxin on root elongation not mediated by ethylene. Plant Physiology 91: 310-314.

ELIASSON L, and BOLLMARK M. 1988. Ethylene as a possible mediator of light-induced inhibition of root growth. Physiologia Plantarum 72: 605-609.

EVANS ML, ISHIKAWA H, and ESTELLE MA.1994. Responses of Arabidopsis roots to auxin studied with high temporal resolution: Comparison of wild type and auxin-response mutants. Planta 194: 215-222.

GASPAR TH, KEEVERS C, FAIVRE-RAMPANT O, CRÈVECOEUR M, PENEL CL, GREPPIN H, and DOMMES J. 2003. Changing concepts in plant hormone action. In Vitro Cell and Developmental Biology - Plant 39: 85-106.

HANSEN H, and GROSSMANN K. 2000. Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition. Plant Physiology 124: 1437-1448.

JACKSON MB. 1991. Ethylene in root growth and development. In: Matoo AK and Suttle JC [eds.], The Plant Hormone Ethylene, 159-181. CRC Press, Boca Raton.

JOO S, SEO YS, KIM SM, HONG DK, PARK KY, and KIM WT. 2006. Brassinosteroid induction of AtACS4 encoding an auxinresponsive 1-aminocyclopropane-1-carboxylate synthase 4 in Arabidopsis seedlings. Physiologia Plantarum 126: 592-604.

KLEE HJ, and ROMANO CP. 1994. The roles of phytohormones in development as studied in transgenic plants. Critical Review Plant Science 13: 311-324.

LEE JS, CHANG WK, and EVANS ML. 1990. Effects of ethylene on the kinetics of curvature and auxin redistribution in gravistimulated roots of Zea mays. Plant Physiology 94: 1770-1775.

LI JS, DAI XH, and ZHAO YD. 2006. A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis. Plant Physiology 140: 899-908.

LÜTHEN H, and BÖTTGER M. 1988. Kinetic of proton secretion and growth in maize roots: action of various plant growth effectors. Plant Science 54: 37-43.

MULKEY TJ, KUZMANOFF KM, and EVANS ML. 1982. Promotion of growth and hydrogen ion efflux by auxin in roots of maize pretreated with ethylene biosynthesis inhibitors. Plant Physiology 70: 186-188.

PITTS RJ, CERNAC A, and ESTELLE M. 1998. Auxin and ethylene promote root hair elongation in Arabidopsis. Plant Journal 16: 553-560.

RAHMAN A, AMAKAWA T, GOTO N, and TSURUMI S. 2001. Auxin is a positive regulator for ethylene-mediated response in the growth of Arabidopsis roots. Plant Cell Physiology 42: 301-307.

RAUSER WE, and HORTON RF. 1975. Rapid effect of indoleacetic acid and ethylene on the growth of intact pea roots. Plant Physiology 55: 443-447.

RODRIGUES-POUSADA R, VAN CAENEGHEM W, CHAUVAUX N, VAN ONEKELEN H, VAN MONTAGU M, and VAN DER STRAETEN D.1999. Hormonal cross-talk regulates the Arabidopsis thaliana 1-aminocyclopropane-1-carboxylate synthase gene 1 in a developmental and tissue-dependent manner. Physiologia Plantarum 105: 312-320.

RUZICKA K, LJUNG K, VANNESTE S, PODHORSKÁ R, BEECKMAN T, and FRIML J, BENKOVÁ E. 2007. Ethylene regulates root growth through effects on auxin biosynthesis and transport-dependent auxin distribution. Plant Cell 19: 2197-2212.

STEPANOVA AN, YUN J, LIKHACHEVA AV, and ALONSO JM. 2007. Multilevel Interactions between ethylene and auxin in Arabidopsis roots. Plant Cell 19: 2169-2185.

SWARUP R, PERRY P, HAGENBEEK D, VAN DER STRAETEN D, BEEMSTER GTS, SANDBERG G, BHALERAO R, LJUNG K, and BENNETT M. 2007. Ethylene upregulates auxin biosynthesis in Arabidopsis seedlings to enhance inhibition of root cell elongation. Plant Cell 19: 2186-2196.

WANG NN, SHIH MC, and LI N. 2005. The GUS reporter aided analysis of the promoter activities of Arabidopsis ACC synthase genes AtACS4, AtACS5, and AtACS7 induced by hormones and stresses. Journal of Experimental Botany 56: 909-920.

WHALEN MC, and FELDMAN LJ. 1988. The effect of ethylene on root growth of Zea mays seedlings. Canadian Journal of Botany 66: 719-723.

YAMADA T, MARUBASHI W, NAKAMURA T, and NIWA M. 2001. Possible involvement of auxin-induced ethylene in an apoptotic cell death during temperature-sensitive lethality expressed by hybrid between Nicotiana glutinosa and N. repanda. Plant Cell Physiology 42: 923-930.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

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