Effect of UV-B Radiation on Antioxidative Enzyme Activity in Cucumber Cotyledons

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Effect of UV-B Radiation on Antioxidative Enzyme Activity in Cucumber Cotyledons

Ultraviolet-B (UV-B) radiation negatively affects plant cells, causing reactive oxygen species (ROS) to be generated. To study the effects of increased UV-B exposure on antioxidant processes, we exposed germination-stage cucumber seedlings to increased ultraviolet radiation and analyzed hydrogen peroxide content and the activity of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX) and syringaldazine peroxidase (SPX). Enzymatic antioxidant system activity generally increased after UV-B supplementation. Activation of CAT, GPX and SPX in cucumber cotyledons correlated positively with increases in SOD activity and hydrogen peroxide content. The results indicate that ROS accumulated despite higher engagement of the enzymatic antioxidant system, and that elevated UV-B radiation triggered oxidative stress in the cucumber cotyledons.

Agrawal SB, and Rathore D. 2007. Changes in oxidative stress defense system in wheat (Triticum aestivum L.) and mung bean (Vigna radiata L.) cultivars grown with and without mineral nutrients and irradiated by supplemental ultraviolet-B. Environmental and Experimental Botany 59: 21-33.

Agrawal SB, Singh S, and Agrawal M. 2009. Ultraviolet-B induced changes in gene expression and antioxidants in plants. Advances in Botanical Research 52: 47-86.

Alscher RG, Erturk N, and Heath LS. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plant. Journal of Experimental Botany 53: 1331-1341.

Asada K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annual Review of Plant Physiology and Plant Molecular Biology 50: 601-639.

Balakumar T, Gayathri B, and Anbudurai PR. 1997. Oxidative stress injury in tomato plants induced by supplemental UV-B radiation. Biologia Plantarum 39: 215-221.

Baumbusch LO, Eiblmeier M, Schnitzler JP, Heller W, Sandermann H, and Polle JA. 1998. Interactive effects of ozone low UV-B radiation on antioxidants in spruce (Picea abies) and pine (Pinus silvestris) needles. Physiologia Plantarum 104: 248-254.

Beauchamp C, and Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-278.

Bolkhina O, Virolainen E, and Fagerstedt KV. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany 91: 179-194.

Bolwell GP, and Wojtaszek P. 1997. Mechanisms for the generation of reactive oxygen species in plant defence - a broad perspective. Physiological and Molecular Plant Pathology 51: 347-366.

Bolwell GP, Bindschedler LV, Blee KA, Butt VS, Davies DR, Gardner SL, Gerrish C, and Minibayeva F. 2002. The apoplastic oxidative burst in response to biotic stress in plants: a three-component system. Journal of Experimental Botany 53: 1367-1376.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.

Clifford SC, Arndt SK, Corlett JE, Joshi S, Sankhla N, Popp M, Jones HG. 1998. The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.) Journal of Experimental Botany 49: 967-977.

Correia CM, Moutinho Pereira JM, Coutinho JF, Björn LO, and Torres-Pereira JMG. 2005. Ultraviolet-B radiation and nitrogen affect the photosynthesis of maize: a Mediterranean field study. European Journal of Agronomy 22:337-347.

Dai Q, Yan B, Huang S, Liu X, Peng S, Miranda ML, Chavez AQ, Vergara BS, and Olszyk DM. 1997. Response of oxidative stress defense systems in rice (Oryza sativa L.) leaves with supplemental UV-B radiation. Physiologia Plantarum 101: 301-308.

Dat J, Vandenabeele S, Vranova E, van Montagu M, Inze D, and van Breusegem F. 2000. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences 57: 779-795.

Dhindsa RS, Plumb-Dhindsa P, and Thorpe TA. 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decrease levels of superoxide dismutase and catalase. Journal of Experimental Botany 32: 93-101.

Foyer CH, and Noctor G. 2003. Redox sensing and signaling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiologia Plantarum 119: 355-364.

Foyer CH, and Noctor G. 2005. Oxidant and antioxidant signaling in plants: re-evaluation of the concept of oxidative stress in physiological context. Plant, Cell and Environment 29: 1056-1071.

Garciá AL, Fuentes V, and Nicolaś N. 2000. Interactive effect of nitrogen and long-term moderate water stress on water relations in tomato (Lycopersicon esculentum Mill.) plants. Journal of Plant Physiology 156: 563-566.

Hammerschmidt R, Nuckles EM, and Kuc J. 1982. Association of enhanced peroxidase activity with induced systemic resistance of cucumber to Colleotrichum lagenarium. Physiological Plant Pathology 20: 73-82.

Han C, Liu Q, and Yang Y. 2009. Short-term effects of experimental warming and enhanced ultraviolet-B radiation on photosynthesis and antioxidant defense of Picea asperata seedlings. Plant Growth Regulation 58: 153-162.

Imberty A, Goldberg R, and Catesson AM. 1985. Isolation and characterization of Populus isoperoxidases involved in the last step of lignin formation. Planta 164: 221-226.

Jain K, Kataria S, and Guruprasad KN. 2004. Oxyradicals under UV-B stress and their quenching by antioxidants. Indian Journal of Biochemistry and Biophysics 42: 884-892.

Kakani VG, Reddy KR, Zhao D, and Sailaja K. 2003. Field crops responses to ultraviolet-B radiation: a review. Agricultural and Forest Meteorology 120: 191-218.

Kataria S, Jain K, and Guruprasad KN. 2007. UV-B induced changes in antioxidant enzymes and their isoforms in cucumber (Cucumis sativus L.) cotyledons. Indian Journal of Biochemistry and Biophysics 44: 31-37.

Kubiš J, and Rybus-Zając M. 2008. Drought and excess UV-B irradiation differentially alter the antioxidant system in cucumber leaves. Acta Biologica Cracoviensia Series Botanica 50: 35-41

Kubo A, Aono M, Nakalima N, Saji H, Tanaka K, and Kondo N. 1999. Differential responses in activity of antioxidant enzymes to different environmental stresses in Arabidopsis thaliana. Journal of Plant Research 112: 279-290.

Lamb C, and Dixon RA. 1997. The oxidative burst in plant disease resistance. Annual Review Plant Physiology and Plant Molecular Biology 48: 251-275.

Langebartels CH, Wohlgemyth H, Kschieschan S, Grün S, and Sandermann H. 2002. Oxidative burst and cell death in ozone-exposed plants. Plant Physiology and Biochemistry 40: 567-575.

Lei Z, Mingyu S, Xiao W, Chao L, Chunxiang Q, Liang C, Hao H, Xiaoqing L, and Fashui H. 2008. Antioxidant stress is promoted by nano-anatase in spinach chloroplasts under UV-B radiation. Biological Trace Element Research 121: 69-79.

Madronich S, Mckenzie LO, Björn LO, and Caldwell MM. 1998. Changes in biologically active ultraviolet radiation reaching the earth's surface. Journal of Phytochemistry and Photobiology. B, Biology 46: 5-19.

Mazza CA, Battista D, Zima AM, Szwarcberg-Bracchitta M, Giordano CV, Acevedo A, and Scopel AL. 1999. The effects of solar ultraviolet-B radiation on the growth and yield of barley are accompanied by increased DNA damage and antioxidant response. Plant Cell Environmental 22: 61-70.

Messner B, and Boll. 1994. Cell suspension cultures of spruce (P. abies): inactivation of extracellular enzymes by fungal elicitor-induced transient release of hydrogen peroxide (oxidative burst). Plant Cell, Tissue and Organ Culture 39: 69-78.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410

Neill SJ, Desikan R, Clarke A, and Hancock J. 1999. H2O2 signaling in plant cells. In: Plant Responses to Environmental Stress. BIOS Scientific Publishers, Oxford 8: 59-64.

Neill SJ, Desikan R, and Hancock J. 2002. Hydrogen peroxide signaling. Current Opinion in Plant Biology 5: 388-395.

Panagopoulos I, Bornman F, and Björn LO. 1990. Effect of ultraviolet radiation and visible light on growth, fluorescence induction, ultra weak luminescence and peroxidase activity in sugar beat plants. Journal of Photochemistry and Photobiology, B: Biology 8: 73-87.

Passardi F, Longet D, Penel C, and Dunand C. 2004. The class III peroxidase multigenic family in rice and its evolution in land plants. Phytochemistry 65: 1879-1893.

Rybus-Zając M. 2005. Oxidative stress generation in Taxus baccata leaves affected by Pestalotiopsis funerea Desm. under different light conditions. Dendrobiology 54: 51-56.

Rao MV, Poliyath G, and Ormrod D. 1996. Ultraviolet and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.Plant Physiology 110: 125-136.

Scandalios JG. 2002. The rise of ROS. Trends in Biochemical Sciences 27: 483-486.

Tang K, Zhan JC, Yang HR, and Huang WD. 2010. Changes of resveratrol and antioxidant enzymes during UV-induced plant defense response in peanut seedlings. Journal of Plant Physiology 167: 95-102.

UNEP. 2002. Executive summary. Final of UNEP/WMO Scientific Assessment of Ozone Depletion: 2002. Prepared by the Scientific Assessment Panel of the Montreal Protocol on Substances that Deplete the Ozone Layer. UNEP, Nairobi (released 23 August 2002).

van Breusegem F, Vranova E, Dat JF, and Inze D. 2001 The role of active oxygen species in plant signal transduction. Plant Science 161: 404-414.

Veljovic-Jovanovic S, Noctor G, and Foyer CH. 2002. Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate. Plant Physiology and Biochemistry 40: 501-507.

Watanabe K, Yamada N, and Takeuchi Y. 2006. Oxidative DNA damage in cucumber cotyledons irradiated with ultraviolet light. Journal of Plant Research 119: 239-246.

Yannarelli GG, Noriega GO, Batlle A, Tomaro ML. 2006a. Heme oxygenase up-regulation in ultraviolet-B irradiated soybean plants involves reactive oxygen species. Planta 224: 1154-1162.

Yannarelli GG, Gallego SM, and Tomaro ML. 2006b. Effect of UV-B radiation on the activity and isoforms of enzymes with peroxidase activity in sunflower cotyledons. Environmental and Experimental Botany 56: 174-181.

Acta Biologica Cracoviensia s. Botanica

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