Responses of Root Growth and Protective Enzymes to Copper Stress in Turfgrass

Open access

Responses of Root Growth and Protective Enzymes to Copper Stress in Turfgrass

Root growth and protective enzymes of Festuca arundinacea L. and Lolium perenne L. under Cu stress were investigated in a hydroponic experiment. Cu stress significantly inhibited root growth (root elongation and dry biomass) of both turfgrasses. Malondialdehyde (MDA) content in roots of both turfgrasses markedly increased under copper stress. In F. arundinacea root, superoxide dismutase (SOD) activity increased greatly with increasing Cu concentration; peroxidase (POD) activity increased at low Cu level and decreased at high Cu level. Increased MDA content indicated the formation of free radicals under Cu stress, while increased SOD activity pointed to the operation of a scavenging mechanism. In roots of L. perenne, however, SOD and POD were not activated by copper. These results demonstrate that turfgrass cultivars clearly differ in tolerance to Cu stress, and that the tolerance depends largely on the enhanced activity of its antioxidant system.

Alaoui-Sossé B, Genet P, Vinit-Dunand F, Toussaint ML, Epron D, and Badot PM. 2004. Effect of copper on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Science 166: 1213-1218.

Bradley R, Burt AJ, and Read DJ. 1981. Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris.Nature 292: 335-337.

Choudhary M, Jetley UK, Khan AM, Zutshi S, and Fatma T. 2007. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5. Ecotoxicol-ogy and Environmental Safety 66: 204-209.

Demirevska-Kepova K, Simova-Stoilova L, Stoyanova Z, Hölzer R, and Feller U. 2004. Biochemical changes in barley plants after excessive supply of copper and manganese. Environmental and Experimental Botany 52: 253-266.

Dietz KJ, Bair M, and Krämer U. 1999. Free radicals and reactive oxygen species as mediators of heavy metals toxicity in plants. In: Prasad MNV and Hagemeyer J [ed.], Heavy Metal Stress in Plants from Molecules to Ecosystems, 73-97. Springer-Verlag, Berlin.

Fernandes JC, and Henriques FS. 1991. Biochemical, physiological, and structural effect of excess copper in plants. Botanical Review 57: 246-273.

Groppa MD, Zawoznik MS, Tomaro ML, and Benavides MP. 2008. Inhibition of root growth and polyamine metabolism in Sunflower (Helianthus annuus) seedlings under cadmium and copper stress. Biological Trace Element Research 126: 246-256.

Hall JL. 2002. Cellular mechanisms for heavy metal detoxification and tolerance. Journal of Experimental Botany 53: 1-11.

Hu KD, Hu LY, Liyh, Zhang FQ, and Zhang H. 2007. Protective roles of nitric oxide on germination and antioxidant metabolism in wheat seeds under copper stress. Plant Growth Regulation 53: 173-183.

Ke WS, Xiong ZT, XIE MJ, and Luo Q. 2007. Accumulation, subcellular localization and ecophysiological responses to copper stress. Plant and Soil 292: 291-304.

Khatun S, Ali MB, Hahn EJ, and Paek KY. 2008. Copper toxicity in Withania somnifera: growth and antioxidant enzymes. Environmental and Experimental Botany 64: 279-285.

Kováčik J, Klejdus B, Hedbavny J, Štork F, and Bačkor M. 2009. Comparison of cadmium and copper effect on phenolic metabolism, mineral nutrients and stress-related parameters in Matricaria chamomilla plants. Plant and Soil 320: 231-242.

Liang YC, Hu F, Yang MC, and Yu JH. 2003. Antioxidative defenses and water deficit-induced oxidative damage in rice (Oryza sativa L.) growing on non-flooded paddy soils with ground mulching. Plant and Soil 257: 407-416.

Liu J, and Xiong ZT. 2005. Differences in accumulation and physiological response to copper stress in three populations of Elsholtzia haichowensis S. Water Air and Soil Pollution 168: 5-16.

Liu WZ, and Sun DL. 1985. Effects of copper on growth, CAT and IAA oxidase activities in cucumber seedling. Plant Physiology Communications (3): 22-24.

Lombardi L, and Sebastiani L. 2005. Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants. Plant Science 168: 797-802.

Luna CM, Gonzalez VS, and Trippi VS. 1994. Oxidative damage caused by excess copper in oat leaves. Plant and Cell Physiology 35: 11-15.

Madejón P, Ramírez-Benítez JE, Corrales I, Barceló J, and Poschenrieder C. 2009. Copper-induced oxidative damage and enhanced antioxidant defenses in root apex of maize cultivars differing in Cu tolerance. Environmental and Experimental Botany 67: 415-420.

Maksymiec W, and Krupa Z. 2007. Effects of methyl jasmonate and excess copper on root and leaf growth. Biologia Plantarum 51: 322-326.

Meloni DA, Oliva MA, Martinez CA, and Cambraia J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environmental and Experimental Botany 49: 69-76.

Mittler R, Vanderauwera S, Gollery M, and Breusegem FV. 2004. Abiotic stress series. Reactive oxygen gene network of plants. Trends in Plant Science 9: 490-498.

Monferrán MV, Sánchez Agudo JA, Pignata ML, and Wunderlin DA. 2009. Copper-induced response of physiological parameters and antioxidant enzymes in the aquatic macrophyte Potamogeton pusillus.Environmental Pollution 157: 2570-2576.

Monnet F, Bordas F, Deluchat V, and Baudu M. 2006. Toxicity of copper excess on the lichen Dermatocarpon luridum: Antioxidant enzyme activities. Chemosphere 65: 1806-1813.

Ouzounidou G, Giamparova M, Moustakas M, and Karataglis S. 1995. Responses of maize (Zea mays L.) plants to copper stress. I. Growth. Environmental and Experimental Botany 35: 167-176.

Ozden M, Demirel U, and Kahraman A. 2009. Effects of praline on antioxidant system in leaves of grapevine (Vitis Vinifera L.) exposed to oxidative stress by H2O2. Scientia Horticulturae 119: 163-168.

Panda SK. 2008. Impact of copper on reactive oxygen species, lipid peroxidation and antioxidants in Lemna minor.Biologia Plantarum 52: 561-564.

Scandalios JG. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101: 7-12.

Shalata A, Mittova V, Volokita M, Guy M, and Tal M. 2001. Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: the root antioxidative system. Physiologia Plantarum 112: 487-494.

Sundar D, Perianayaguy B, and Reddy AR. 2004. Localization of antioxidant enzymes in the cellular compartments of sorghum leaves. Plant Growth Regulation 44: 157-163.

Tanyolaç D, Ekmekçi Y and Ünalan Æ. 2007. Changes in photochemical and antioxidant enzyme activities in maize (Zea mays L.) leaves exposed to excess copper. Chemosphere 67: 89-98.

Teisseire H, and Guy V. 2000. Copper-induced changes in antioxidant enzymes activities in fronds of duckweed (Lemna minor). Plant Science 153: 65-72.

Wang SH. 2005. Different responses to Cu stress of different Brassica juncea L species. Journal of Biology 22: 30-32.

Weckx JEJ, and Clijsters HMM. 1996. Oxidative damage and defense mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper. Physiologia Plantarum 96: 506-512.

Wei L, Luo CL, Li XD, and Shen ZG. 2008. Copper accumulation and tolerance in Chrysanthemum coronarium L. and Sorghum sudanense L. Archives of Environmental Contamination and Toxicology 55: 238-246.

Zhang LH, Li PJ, Li XM, Meng XL, and Xu CB. 2005. Effects of cadmium stress on the growth and physiological characteristics of wheat seedlings. Chinese Journal of Ecology 24: 458-460.

Zhang ZL, and Qu WJ. 2003. The Experimental Guide for Plant Physiology. Higher Education Press, Beijing.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2016: 0.491
5-year IMPACT FACTOR: 0.787

CiteScore 2016: 0.51

SCImago Journal Rank (SJR) 2016: 0.242
Source Normalized Impact per Paper (SNIP) 2016: 0.264

Ministry of Science and Higher Education: 20 points

Cited By


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 103 103 15
PDF Downloads 35 35 5