Norway Spruce (Picea abies [L.] Karst.) Provenance Variation in Autumn Cold Hardiness: Adaptation or Acclimation?
We tested autumn frost hardiness in three Norway spruce (Picea abies [L.] Karst.) provenances originating from different altitudes at two trial plots in Slovakia (Vel'ký Lom at 450 m a.s.l., Mútne-Zákamenné at 1,250 m a.s.l.) in a spinoff experiment of the IUFRO 1964/68 Inventory Provenance Experiment with Norway spruce. Two approaches were used to assess hardiness: the electrolyte-leakage method based on artificial freezing, and measurements of chlorophyll a fluorescence parameters. The frost injury index at -20°C differed between provenances, with a significant provenance-by-plot interaction. In material from the lower-altitude Vel'ký Lom trial plot, the high-elevation TANAP provenance exhibited much lower frost injury than the middle-elevation Habovka and low-elevation Beňuš provenances. In material from the high-altitude Mútne-Zákamenné trial plot, all three tested provenances showed approximately the same degree of frost injury. At -80°C no differences between provenances were observed, and the trees growing at the high-elevation site exhibited lower average frost injury than the trees at Vel'ký Lom. Most parameters of the kinetics of chlorophyll a fluorescence followed the same trends as frost injury, and differed significantly between plots. We suggest that the observed differences resulted from acclimation of trees to the conditions of the trial plots rather than from adaptation through natural selection.
Aitken SN, and Adams WT. 1996. Genetics of fall and winter cold hardiness of coastal Douglas-fir in Oregon. Canadian Journal of Forest Research 26: 1828-1837.
Aitken SN, Adams WT, Schermann N, and Fuchigami LH. 1996. Family variation for fall cold hardiness in two Washington populations of coastal Douglas-fir (Pseudotsuga menziesii var menziesii (Mirb) Franco). Forest Ecology and Management 80: 187-195.
Aldrete A, Mexal JG, and Burr KE. 2008. Seedling cold hardiness, bud set, and bud break in nine provenances of Pinus greggii Engelm. Forest Ecology and Management 255: 3672-3676.
Andersson B, and Fedorkov A. 2004. Longitudinal differences in Scots pine frost hardiness. Silvae Genetica 53: 76-80.
Anekonda TS, Adams WT, Aitken SN, Neale DB, Jermstad KD, and Wheeler NC. 2000. Genetics of cold hardiness in a cloned full-sib family of coastal Douglas-fir. Canadian Journal of Forest Research 30: 837-840.
Beuker E, Valtonen E, and Repo T. 1998. Seasonal variation in the frost hardiness of Scots pine and Norway spruce in old provenance experiments in Finland. Forest Ecology and Management 107: 87-98.
Bower AD, and Aitken SN. 2006. Geographic and seasonal variation in cold hardiness of whitebark pine. Canadian Journal of Forest Research 36: 1842-1850.
Cannell MGR, Tabbush PM, Deans JD, Hollingsworth MK, Sheppard LJ, Philipson JJ, and Murray MB. 1990. Sitka spruce and Douglas-fir seedlings in the nursery and in cold storage - root growth potential, carbohydrate content, dormancy, frost hardiness and mitotic index. Forestry 63: 9-27.
Daehlen AG, Johnsen Ø, and Kohmann K. 1995. Høstfrostherdighet hos unge granplanter fra norske provenienser og frøplantasjer. Rapport frå Skogforsk 1/95, 24 pp.
Eckert AJ, Wegrzyn JL, Pande B, Jermstad KD, Lee JM, Liechty JD, Tearse BR, Krutovsky KV, and Neale DB. 2009. Multilocus patterns of nucleotide diversity and divergence reveal positive selection at candidate genes related to cold hardiness in coastal Douglas fir (Pseudotsuga menziesii var. menziesii). Genetics 183: 289-298.
Eysteinsson T, Karlman L, Fries A, Martinsson O, and Skulason B. 2009. Variation in spring and autumn frost tolerance among provenances of Russian larches (Larix Mill.). Scandinavian Journal of Forest Research 24: 100-110.
Flint HL, Boyce BR, and Beattie DJ. 1967. Index of injury - a useful expression of freezing injury to plant tissues as determined by the electrolytic method. Canadian Journal of Plant Science 47: 229-230.
Garcia-Banuelos ML, Moreno LV, Winzerling J, Orozco JA, and Gardea AA. 2008. Winter metabolism in deciduous trees: mechanisms, genes and associated proteins. Revista Fitotecnica Mexicana 31: 295-308.
Holliday JA, Ralph SG, White R, Bohlmann J, and Aitken SN. 2008. Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis). New Phytologist 178: 103-122.
Jensen JS, and Deans JD. 2004. Late autumn frost resistance of twelve north European provenances of Quercus species. Scandinavian Journal of Forest Research 19: 390-399.
Johnsen Ø, Daehlen OG, Østreng G, and Skrøppa T. 2005. Daylength and temperature during seed production interactively affect adaptive performance of Picea abies progenies. New Phytologist 168: 589-596.
Johnsen Ø, and Østreng G. 1994. Effects of plus tree selection and seed orchard environment on progenies of Picea abies. Canadian Journal of Forest Research 24: 32-38.
Johnsen Ø, and Skrøppa T. 2000. Provenances and families show different patterns of relationship between bud set and frost hardiness in Picea abies.Canadian Journal of Forest Research 30: 1858-1866.
Joosen RVL, Lammers M, Balk PA, Bronnum P, Konings MCJM, Perks M, Stattin E, van Wordragen MF, and van der Geest AHM. 2006. Correlating gene expression to physiological parameters and environmental conditions during cold acclimation of Pinus sylvestris, identification of molecular markers using cDNA microarrays. Tree Physiology 26: 1297-1313.
Kalberer SR, Wisniewski M, and Arora R. 2006. Deacclimation and reacclimation of cold-hardy plants: Current understanding and emerging concepts. Plant Science 171: 3-16.
Kozlowski TT, and Pallardy SG. 2002. Acclimation and adaptive responses of woody plants to environmental stresses. Botanical Review 68: 270-334.
Krutzsch P. 1974. The IUFRO 1964/69 provenance test with Norway spruce (Picea abies (L.) Karst.). Silvae Genetica 23: 58-62.
Kvaalen H, and Johnsen Ø, 2008. Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytologist 177: 49-59.
Li CY, Junttila O, Heino P, and Palva ET. 2004. Low temperature sensing in silver birch (Betula pendula Roth) ecotypes. Plant Science 167: 165-171.
Lu PX, Joyce DG, and Sinclair RW. 2003. Geographic variation in cold hardiness among eastern white pine (Pinus strobus L.) provenances in Ontario. Forest Ecology and Management 178: 329-340.
Mahalovich MF, Burr KE, and Foushee DL. 2006. Whitebark pine germination, rust resistance, and cold hardiness among seed sources in the inland northwest: Planting strategies for restoration. USDA Forest Service Rocky Mountain Research Station Proceedings 43: 91-101.
Maxwell K, and Johnson GN. 2000. Chlorophyll fluorescence - a practical guide. Journal of Experimental Botany 51: 659-668.
Nielsen CCN, and Rasmussen HN. 2009. Frost hardening and dehardening in Abies procera and other conifers under differing temperature regimes and warm-spell treatments. Forestry 82: 43-59.
O'Neill GA, Aitken SN, and Adams WT. 2000. Genetic selection for cold hardiness in coastal Douglas fir seedlings and saplings. Canadian Journal of Forest Research 30: 1799-1807.
Repo T. 1992. Seasonal changes of cold hardiness in Picea abies and Pinus sylvestris in Finland. Canadian Journal of Forest Research 22: 1949-1957.
Repo T, Leinonen I, Wang K-Y, and Hänninen H. 2006. Relation between photosynthetic capacity and cold hardiness in Scots pine. Physiologia Plantarum 126: 224-231.
Repo T, Zhang G, Ryyppö A, Rikala R, and Vuorinen M. 2000. The relation between growth cessation and frost hardening in Scots pines of different origins. Trees 14: 456-464.
Saenz-Romero C, and Tapia-Olivares BL. 2008. Genetic variation in frost damage and seed zone delineation within an altitudinal transect of Pinus devoniana (P. michoacana) in Mexico. Silvae Genetica 57: 165-170.
Sano H. 2002. DNA methylation and Lamarckian inheritance. Proceedings of the Japan Academy Series B - Physical and Biological Sciences 78: 293-298.
SAS. 1988. SAS/STAT® User's Guide, Release 6.03 edition. SAS Institute, Cary
Savolainen O, Pyhajärvi T, and Knurr T. 2007. Gene flow and local adaptation in trees. Annual Review of Ecology, Evolution and Systematics 38: 595-619.
Schrader JA, and Graves WR. 2003. Phenology and depth of cold acclimation in the three subspecies of Alnus maritima. Journal of the American Society for Horticultural Science 128: 330-336.
Skrøppa T, Tollefsrud MM, Sperisen C, and Johnsen Ø. 2009. Rapid change in adaptive performance from one generation to the next in Picea abies - Central European trees in a Nordic environment. Tree Genetics and Genomes 6: 93-99.
Smallwood M, and Bowles DJ. 2002. Plants in a cold climate. Philosophical Transactions of the Royal Society of London Series B - Biological Sciences 357: 831-846.
Steward N, Ito M, Yamaguchi Y, Koizumi N, and Sano H. 2002. Periodic DNA methylation in maize nucleosomes and demethylation by environmental stress. Journal of Biological Chemistry 277: 37741-37746.
Taulavuori K, Taulavuori E, Sarjala T, Savonen EM, Pietilainen P, Lahdesmaki P, and Laine K. 2000. In vivo chlorophyll fluorescence is not always a good indicator of cold hardiness. Journal of Plant Physiology 157: 227-229.
Thomas BR, and Lester DT. 1992. An examination of regional, provenance, and family variation in cold hardiness of Pinus monticola. Canadian Journal of Forest Research 22: 1917-1921.
Weiser CJ. 1970. Cold resistance and injury in woody plants. Science 169: 1269-1278.