Overexpression of Arabidopsis AtmiR408 Gene in Tobacco

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Overexpression of Arabidopsis AtmiR408 Gene in Tobacco

MicroRNA miR408 is predicted to target the transcripts for the copper proteins plantacyanin and laccase in Arabidopsis. In this study it was found that miR408 is present in tobacco also. Its accumulation was positively induced by copper deficiency, providing additional evidence for the important role of miR408 in plant responses to nutriment conditions. To examine the functions of miR408 in tobacco, transgenic tobacco lines containing the Arabidopsis AtmiR408 gene were created. Two transgenic lines with miR408 oversupply were chosen for functional characterization. The results showed that miR408 oversupply down-regulated the activities of antioxidant enzymes such as SOD, POD and CAT. As a result, transgenic lines exhibited altered chlorophyll content and seedling root growth, especially under high copper conditions. This suggests that miR408 accumulates in response to copper deficiency and influences plant growth by regulating antioxidant enzymes. It may be that miR408 exists and functions in tobacco in a conserved way similarly to miR408 in Arabidopsis.

Abdel-Ghany SE, and Pilon M. 2008. MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis. The Journal of Biological Chemistry 283: 15932-15945.

Bartel DP. 2004. MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116: 281-297.

Dean JF, and Eriksson KE. 1994. Laccase and the deposition of lignin in vascular plants. Holzforschung 48: 21-33.

Dong J, Kim ST, and Lord EM. 2005. Plantacyanin plays a role in reproduction in Arabidopsis.Plant Physiology 138: 778-789.

Fahlgren N, Howell MH, Kasschau KD, Elisabeth JC, Sullivan CM, Cumbie JS, Givan SA, Law TF, Grant SR, Dangl JL, and Carrington JC. 2007. High-throughput sequencing of Arabidopsis microRNAs: evidence for frequent birth and death of MIRNA genes. PLoS ONE 2: e219.

Hoopes JT, and Dean JFD. 2004. Ferroxidase activity in a plant multicopper oxidase. Plant Physiology and Biochemistry 42: 27-33.

Jones-Rhoades MW, and Bartel DP. Bartel B. 2006. MicroRNAs and their regulatory roles in plants. Annual Review of Plant Biology 57: 19-53.

LaFayette PR, Eriksson KE, and Dean JF. 1999. Characterization and heterologous expression of laccase cDNAs from xylem tissues of yellow-poplar (Liriodendron tulipifera). Plant Molecular Biology 40: 23-35.

Liang M, Davis E, Gardner D, Cai X, and Wu Y. 2006. Involvement of AtLAC15 in lignin synthesis in seeds and in root elongation of Arabidopsis.Planta 224: 1185-1196.

McCaig BC, Meagher RB, and Dean JFD. 2005. Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana.Planta 221: 619-636.

Prochazkova D, Sairam RK, Srivastava GC, and Singh DV. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Science 161: 765-771.

Ranocha P, Chabannes M, Chamayou S, Danoun S, Jauneau A, Boudet AM, and Goffner D. 2002. Laccase down-regulation causes alterations in phenolic metabolism and cell wall structure in poplar. Plant Physiology 129: 145-155.

Sunkar R, and Zhu JK. 2004. Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis.Plant Cell 16: 2001-2019.

Weigel M, Varotto C, Pesaresi P, Finazzi G, Rappaport F, Salamini F, and Leister D. 2003. Plastocyanin is indispensable for photosynthetic electron flow in Arabidopsis thaliana.The Journal of Biological Chemistry 278: 31286-31289.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

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