Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Open access

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl2, and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.

Agrawal GK, Rakwal R, and Jwa NS. 2000. Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals. Biochemical and Biophysical Research Communications 278: 290-298.

Andjelkovic V, and Thompson R. 2006. Changes in gene expression in maize kernel in response to water and salt stress. Plant Cell Reports 25: 71-79.

Beauchamp VB, Stromberg JC, and Stutz JC. 2005. Interactions between Tamarix ramosissima (saltcedar), Populus fremontii (cottonwood), and mycorrhizal fungi: Effects on seedling growth and plant species coexistence. Plant and Soil 275: 221-231.

Breiteneder H, Hoffmann-Sommergruber K, O'Riordain G. Susani M, Ahorn H, Ebner C, Kraft D, and Scheiner O. 1995. Molecular characterization of Api g 1, the major allergen of celery (Apium graveolens), and its immunological and structural relationships to a group of 17-kDa tree pollen allergens. European Journal of Biochemistry 233: 484-489.

Christensen AB, Cho BH, Naesby M, Gregersen PL, Brandt J, Madriz-Ordenana K, Collinge DB, and Thordal-Christensen H. 2002. The molecular characterisation of the two barley proteins establishes the novel PR-17 family of pathogenesis-related protein. Moloecular Plant Pathology 3: 134-144.

Colditz F, Nyamsuren O, Niehaus K, Eubel H, Braun HP, and Krajinski F. 2004. Proteomic approach: identification of Medicago truncatula proteins induced in roots after infection with the pathogenic oomycete Aphanomyces euteiches.Plant Molecular Biology 55: 109-120.

Ding CK, Wang CY, Gross KC, and Smith DL. 2002. Jasmonate and salicylate induce the expression of pathogenesis-related-protein genes and increase resistance to chilling injury in tomato fruit. Planta 214: 895-901.

Dubos C, and Plomion C. 2001. Drought differentially affects expression of a PR-10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings. Jounal of Experimental Botany 52: 1143-1144.

El-Kereamy A, Jayasankar S, Taheri A, Errampalli D, and Paliyath G. 2009. Expression analysis of a plum pathogenesis related protein 10 (PR10) during brown rot infection. Plant Cell Reports 28: 95-102.

Evelin H, Kapoor R, and Giri B. 2009. Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot. 104:1263-1280.

Fujimoto Y, Nagata R, Fukasawa H, Yano K, Azuma M, Iida A. et al. 1998. Purification and cDNA cloning of cytokine inspecific binding protein from mung bean (Vigna radiata). European Journal of Biochemistry 258: 794-802.

Gonneau M, Pagant S, Brun F, and Laloue M. 2001. Photoaffinity labeling with the cytokinin agonist azido-CPPU of a 34 kDa peptide of the intracellular pathogenesis related protein family in the moss Physcomitrella patens. Plant Molecular Biology 46: 539-548.

Hmida-Sayari A, Costa A, Leone A, Jaoua S, and Gargouri-Bouzid R. 2005. Identification of salt stress-induced transcripts in potato leaves by cDNA-AFLP. Molecular Biotechnology 30: 31-40.

Hoffmann-Sommergruber K, Vanek-Krebitz M, Radauer C, Wen J, Ferreira F, Scheiner O, and Breiteneder H. 1997. Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions. Gene 197: 91-100.

Hu X. Jiang M, Zhang J, Zhang A, LIN F, and Tan M. 2007. Calcium-calmodulin is required for abscisic acid-induced antioxidant defense and functions both upstream and downstream of H2O2 production in leaves of maize (Zea mays) plants. New Phytologyst 173: 27-38.

Huang W, Ma X, Wang Q, Gao Y, Xue Y, Niu X, Yu G, and Liu Y. 2008. Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays). Plant Moecularl Biology 68: 451-463.

Jellouli N, Ben Jouira H, Skouri H, Ghorbel A, Gourgouri A, and Mliki A. 2008. Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress. Plant Physiology 165: 471-481.

Kieffer P, Dommes J, Hoffmann L, Hausman JF, and Renaut J. 2008. Quantitative changes in protein expression of cadmium-exposed poplar plants. Proteomics 8: 2514-2530.

Kim ST, Yu S, Kang YH, Kim SG, Kim JY, Kim SH, and Kang KY. 2008. The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Reports 27: 593-603.

Koistinen KM, Hassinen VH, Gynther PA, Lehestranta SJ, Keinänen SI, Kokko HI, Oksanen EJ, Tervahauta AI, Auriola S, and Kärenlampi SO. 2002. Birch PR-10c is induced by factors causing oxidative stress but appears not to confer tolerance to these agents. New Phytologyst 155: 381-391.

Kore-eda S, Cushman MA, Akselrod I, Bufford D, Fredrickson M, Clark E, and Cushman JC. 2004. Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum.Gene 341: 83-92.

Li H, Wang Y, Jiang J, Liu G, Gao C, and Yang C. 2008. Identification of genes responsive to salt stress on Tamarix hispida roots. Gene. [Epub ahead of print]

Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun X, and Tang K. 2006. A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible. Journal of Plant Physiology 163: 546-556.

Liu JJ, and Ekramoddoullah AK. 2006. The family 10 of plant pathogenesis-related proteins: Their structure, regulation, and function in response to biotic and abiotic stresses. Physiological and Molecular Plant Pathology 68: 3-13.

Livak KJ, and Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(Delta Delta C(T)) Method. Methods 25: 402-408.

Lorenz WW, Sun F, Liang C, Kolychev D, Wang H, Zhao X, Cordonnier-Pratt MM, Pratt LH, and Dean JF. 2006. Water stress-responsive genes in loblolly pine (Pinus taeda) roots identified by analyses of expressed sequence tag libraries. Tree Physiology 26: 1-16.

Moons A, Prinsen E, Bauw G, and Van Montagu M. 1997. Antagonistic effects of abscisic acid and jasmonates on salt stress-inducible transcripts in rice roots. Plant Cell 9: 2243-2259.

Odjakova M, and Hadjiivanova C. 2001. The complexity of pathogen defense in plants. Plant Physiology 27: 101-109.

Onishi M, Tachi H, Kojima T, Shiraiwa M, and Takahara H. 2006. Molecular cloning and characterization of a novel salt-inducible gene encoding an acidic isoform of PR-5 protein in soybean (Glycine max [L.] Merr). Plant Physiology and Biochemistry 44: 574-580.

Park CJ, Kim KJ, Shin R, Park JM, Shin YC, and Paek KH. 2004. Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. Plant 37: 186-198.

Randeep R, Ganesh KA, and Masami Y. 2001. Light-dependent induction of OsPR10 in rice (Oryza sativa L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Science 161: 469-479.

Riggleman RC, Fristensky B, and Hadwiger LA. 1985. The disease resistance response in pea is associated with increased mRNAs. Plant Molecular Biology 4: 81-86.

Sarowar S, Kim YJ, Kim EN, Kim KD, Hwang BK, Islam R, and Shin JS. 2005. Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses. Plant Cell Reports 24: 216-224.

Seo PJ, Lee AK, Xiang F, and Park CM. 2008. Molecular and functional profiling of Arabidopsis pathogenesis-related genes: insights into their roles in salt response of seed germination. Plant Cell Physiology 49: 334-344.

Srivastava S, Emery RJ, Kurepin LV, Reid DM, Fristensky B, and Kav NN. 2006. Pea PR10.1 is a ribonuclease and its transgenic expression elevates cytokinin levels. Plant Growth Regulation 49: 17-25.

Tang DQ, Qian HM, Yu SW, CaoYF, Liao ZH, Zhao LX, Sun XF, Huang DF, and Kexuan T. 2004. cDNA cloning and characterization of a new stress-responsive gene BoRS1 from Brassica oleracea var. acephala.Physiologia Plantarum 121: 578-585.

Ueno O. 1998. Induction of Kranz Anatomy and C4-like biochemical characteristics in a submerged amphibious plant by abscisic acid. Plant Cell 10: 571-584.

van Loon LC, Pierpoint WS, Boller Th, and Conejero V. 1994. Recommendation for naming plant pathogenesis-related proteins. Plant Molecular Biology Reporter 12: 245-264.

van Loon LC, Rep M, and Pieterse CMJ. 2006. Significance of inducible defense-related proteins in infected plants. Annual Review of Phytopathology 44: 1-28.

Wang CS, Huang JC, and Hu JH. 1999. Characterization of two subclasses of PR-10 transcripts in lily anthers and induction of their genes through separate signal transduction pathways. Plant Molecular Biology 40: 807-814.

Wang YC, Bo HX, and Yang CP. 2003. A method for rapid isolation of total RNA from Tamarix and Populous euphratica Oliv. Journal of Northeast Forestry University 31: 99-100.

Wisniewski M, Bassett C, and Arora R. 2004. Distribution and partial characterization of seasonally expressed proteins in different aged shoots and roots of ‘Loring’ peach (Prunus persica). Tree Physiology 24: 339-345.

Xiong L, Ishitani M, Lee H, and Zhu JK. 2001. The Arabidopsis LOS5/ABA3 Locus Encodes a Molybdenum Cofactor Sulfurase and Modulates Cold Stress- and Osmotic Stress-Responsive Gene Expression. Plant Cell 13: 2063-2084.

Yan QJ, Qi XW, Jiang ZQ, Yang SQ, and Han LJ. 2008. Characterization of a pathogenesis-related class10 protein (PR-10) from Astragalus mongholicus with ribonuclease activity. Plant Physiology and Biochemistry 46: 93-99.

Yu XM, Griffith M, and Wiseman SB. 2001. Ethylene induces antifreeze activity in winter rye leaves. Plant Physiology 126: 1132-1140.

Zhu B, Chen TH, and Li PH. 1995. Expression of three osmotin-like protein genes in response to osmotic stress and fungal infection in potato. Plant Molecular Biology 28: 17-26.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

Journal Information

IMPACT FACTOR 2016: 0.491
5-year IMPACT FACTOR: 0.787

CiteScore 2016: 0.51

SCImago Journal Rank (SJR) 2016: 0.242
Source Normalized Impact per Paper (SNIP) 2016: 0.264

Ministry of Science and Higher Education: 20 points


All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 132 132 19
PDF Downloads 47 47 8